Scientists create 'most simple of all organisms'

Bare-essentials bacterial cell with only 473 genes could offer insight into 'minimal basis of life'

WASHINGTON • Scientists on Thursday announced the creation of a synthetic organism stripped down to the bare essentials with the fewest genes needed to survive and multiply, a feat at the microscopic level that may provide big insights on the very nature of life.

Genome research pioneer J. Craig Venter called the bacterial cell that his research team designed and constructed the "most simple of all organisms". While the human genome possesses more than 20,000 genes, the new organism gets by with only 473. "This study is definitely trying to understand a minimal basis of life," said Dr Venter.

But the researchers said that even with such a simple organism, that understanding remained elusive. They noted that even though their organism has so few genes, they were still uncertain about the function of nearly a third of them.The researchers predicted their work would yield practical applications in developing medicines, biochemicals and biofuels and in agriculture.

"Our long-term vision has been to design and build synthetic organisms on demand where you can add in specific functions and predict what the outcome is going to be," said Dr Daniel Gibson, vice-president for DNA technologies at Synthetic Genomics, the company handling commercial applications from the research.

Dr Venter helped map the human genome in 2001 and created the first synthetic cell in 2010 with the same team which conducted the new research. That 2010 achievement, creating a bacterial organism with a man-made genome, demonstrated that genomes can be designed on a computer, made in a laboratory and transplanted into a cell to form a new, self-replicating organism.

Having created that synthetic cell, the researchers set out to engineer a bacterium by removing unessential genes. The goal was to use the fewest genes necessary for the organism to live and reproduce.

Dr Venter said one lesson was that to understand life, it is more important to look across the entirety of a genome, an organism's complete genetic blueprint, rather than at individual genes.

The researchers said they created a minimal cell possessing the smallest genome of any self-replicating organism. Microbiologist Clyde Hutchison, lead author of the study in the journal Science, said the goal is to figure out the functions of all the cell's genes and make a computer model to predict how it would grow and change in different environments or with additional genes. "It's important to realise there is no cell that exists where we know the functions of all the genes," Dr Hutchison said.

The environmental group Friends of the Earth expressed concern about the research, citing the lack of government regulations specific to synthetic biology and gene editing technologies. "Living organisms like bacteria are not machines to be rewired," said Ms Dana Perls, an official of the group. "Not even the scientists know the biological function of 149 of these genes, which raises safety concerns. If we don't fully understand the science, it is more difficult to manage bio-safety concerns."


A version of this article appeared in the print edition of The Straits Times on March 26, 2016, with the headline 'Scientists create 'most simple of all organisms''. Print Edition | Subscribe