SINGAPORE - Joseph Schooling was dethroned in the 100-yard butterfly final at the National Collegiate Athletic Association (NCAA) Division I Men's Swimming and Diving Championships in Indiana, clocking 43.75 seconds to finish second on Friday evening (Saturday morning, Singapore time).
The University of Florida's Caeleb Dressel won the race in an NCAA record of 43.58sec.
Schooling's fellow University of Texas (UT) team-mate Jack Conger was third in 44.35sec.
Singaporean Quah Zheng Wen finished in fifth in 45.06sec.
Schooling and Quah had earlier won their respective heats - two-time defending champion Schooling posting 44.97sec to qualify for the final third overall, while Quah clocked 45.27sec to place fifth.
Quah, 20, also won his 100-yard backstroke heat in 46.33sec, but did not advance to the final.
The University of California, Berkeley freshman is making his NCAA debut at the March 22-25 Championships.
Schooling, a UT junior, won two relay golds (200-yd freestyle, 400-yd medley) and an individual bronze (50-yd freestyle) on Thursday.
The 21-year-old and Quah will swim in the 200-yd butterfly on Saturday (March 25).
Schooling, the two-time defending 200-fly champion, holds the NCAA record of 1:37.97min in the event, and will be gunning for a third straight title.