Zouk came in at No. 3 on the DJ Mag Top 100 clubs list for the first time, while Ce La Vi climbed 13 spots to No. 67.
This was the strongest showing yet for both Singapore clubs.
Zouk, located in Clarke Quay, placed at No. 4 last year in the ranking of the world's best dancefloors by the British music magazine.
The 27-year-old club, which has consistently placed in the top 10 since 2010, remains the highest-ranked club in Asia, ahead of Seoul's Octagon, which dropped from the fifth to seventh spot.
Meanwhile, Ce La Vi, located on the 57th floor of Marina Bay Sands, climbed from No. 90 in 2016 to No. 80 last year and to No. 67 this year.
The results were released on Wednesday, Singapore time.
Brazil's Green Valley claimed the top spot, climbing up two places, while Ibiza's Ushuaia climbed four spots to No. 2.
On breaking into the top three, chief executive officer of Zouk Group Andrew Li says he hopes to "keep up the positive momentum and bring success to our upcoming expansion projects in the pipeline".
As a stalwart of the nightlife scene here, Zouk regularly sees a revolving line-up of some of the biggest names in the dance music world, with the likes of DJs Above & Beyond, Sven Vath and Tiesto gracing the decks in the past year.
Zouk also plans to open an outpost, Zouk Genting, in the coming year.
"Topping the list would be any club's dream and if that dream happens in the near future, it will be a proud moment for Zouk, but more importantly, Singapore, as it'll be primed as the go-to destination for a top-notch clubbing experience," he adds.
Mr Sourav Biswas, general manager of Ce La Vi, says "the only way is up" and hopes to break into the top 50 next year.
"We're proud of the hard work that we've put into making it one of Singapore's best nightlife venues, by upping the quality of service through launching our hospitality training academy, along with our bigger line-up of international guest DJs the past year," he says.
"To jump this high is a validation of our work."
Along with bringing in acts such as Bingo Players, Roger Sanchez and Jazzy Jeff in the past year, Ce La Vi also hosts day-time parties such as the quarterly Sky High Brunch, which will be next held on May 5.
To celebrate the top-three ranking, Zouk will be holding promotions for the next three months, from the first week of next month to July 7.
These include entry at $20 for women and $25 for men from 8 to 10pm on regular club nights, while Zouk members can get one-for-one house pours at the American Express Members Lounge between midnight and 1am.
Zouk will also be holding a celebration party on April 28 featuring its resident DJs.
The first 300 attendees will get complimentary entry before midnight, along with free Zouk memorabilia, while stocks last.