Full-time National Serviceman Chia Wee Kiak, 24, has died from head injuries which he suffered on Sunday, during an accident at the OCBC Cycle Singapore.
Mr Chia, who was a first-timer at the annual ride, hurt himself at Benjamin Sheares Bridge soon after the start of the 59km Super Challenge.
He was brought by ambulance to the Singapore General Hospital where he underwent a 13-hour surgery, and remained in the intensive care unit since.
While investigations are still ongoing it is believed that Mr Chia fell on his own while riding down slope.
Mr Chia leaves behind his parents Han Meng and Adeline, who are in their 50's, and younger sister Jia Yin, 21.
Event's organiser Spectrum Worldwide and OCBC Bank expressed their condolences in a joint statement.
It read: "We are deeply saddened by the passing of Mr Chia Wee Kiak. We would like to express our deepest condolences to the family. Spectrum Worldwide and OCBC will continue to extend all the support needed to Mr Chia's family in this very difficult time."
Over 11,500 people took part in this year's event. The death is the first fatality in the six-year history of the event.