During the song launch held at My First Skool at Whampoa Drive, health minister Khaw Boon Wan joined the pre-schoolers in hand washing and health education lessons.
Copies (CDs) of the song will be distributed to every pre-school and childcare centre here.
Said HPB's chief Lam Pin Woon: 'The Washy Washy Clean song helps children to remember proper hand washing steps in a fun way.
'We hope that it will create a ripple effect in the family, and encourage other family members to adopt good hygiene practices to protect themselves against infectious diseases such as influenza and hand, foot and mouth disease.'